Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IH

Lysyl oxidase (LOX) is an extracellular enzyme that is critical for the normal biosynthesis of collagens and elastin. LOX is synthesized as a 50kD precursor, secreted into the extracellular matrix and subsequently proteolytically processed to a 30kD active enzyme and an 18kD propeptide. LOX also reverts ras-mediated transformation and its expression is down-regulated in human cancers. Most recently, the lysyl oxidase pro-peptide (LOPP) has been associated with tumor suppression, as it inhibits ras-dependent cellular transformation.
Catalog #	L3421-15
Applications	Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunohistochemistry: 0.5-1ug/ml
	Optimal dilutions to be determined by the researcher.
Positive Control	MC3T3-E1 lysate
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	A synthetic peptide derived from an internal region of the mouse protein (residues 78-115).
Specificity	Recognizes the mouse pro-enzyme (50kD) and pro-peptide. The pro-peptide runs at ~18-20kD when not glycosylated and ~35kD on SDS-PAGE when N-glycosylated. Species Crossreactivity: human, rat, primate and porcine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.