| Catalog # | L5999-06A |
| Applications | Suitable for use in Flow Cytometry, Immunohistochemistry, ELISA and Western Blot. Other applications not tested. |
| Recommended Dilutions | |
| Immunohistochemistry: Frozen |
| Flow Cytometry: 10ug/ml. Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. |
| Western Blot: 1:100 |
| Optimal working dilutions to be determined by researcher. |
| Recommended Secondary Reagents | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo |
| I1904-23: IgG, X-Adsorbed (FITC) Pab Gt xMo |
| Recommended Negative Control | I1904-78R: IgG1 Murine Negative Control |
| Hybridoma | Sp20 myeloma cells with spleen cells from Balb/c mice. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H2605 |
| Host | Mouse |
| Source | Firefly |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid, 0.1% sodium azide. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Recombinant luciferase expressed in E. coli. |
| Specificity | Recognizes the luciferase gene of the North American firefly Photinus Pyralis in cells and tissues. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
