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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH IF

The antibody is derived from luciferase of the North American firefly (Photinus pyralis). Luciferase is a 61kD enzyme that catalyzes the reaction of luciferin to generate light after its activation by ATP and oxidative decarboxylation. When expressed in mammalian cells, luciferase molecules produce luminescence in direct proportion to their number in the presence of luciferin and ATP. In this respect, Luciferase is commonly used as a promoter to quantify expression levels from promoters in tissue culture and in vivo.
Catalog #	L5999-27
Applications	Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications have not been tested.
Recommended Dilution	Western Blot: 1-3ug/ml. Observed in mouse heart, liver, kidney, and cortex tissues containing added purified luciferase protein.
	Immunohistochemistry (Frozen Tissue): 15-50ug/ml. Detects tissue specific expression of Luciferase protein in Drosophila embryos using Gal4-UAS system with two different Gal4 drivers.
	Immunofluorescence: 10-30ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	N/A
Clone Type	Monoclonal
Isotype	IgG1
Clone No	6A106
Host	Mouse
Concentration	0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, containing 0.1% sodium azide
Purity	Purified by Protein A chromatography
Immunogen	Luciferase protein from Photinus pyralis
Specificity	Recognizes luciferase. No cross reactivity to mouse heart, NIH3T3, Jurkat, and human fibroblasts
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.