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You are here:Home » Antibodies » Abs to Enzymes » Anti -Luciferase

Anti -Luciferase

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
The antibody is derived from luciferase of the North American firefly (Photinus pyralis). Luciferase is a 61kD enzyme that catalyzes the reaction of luciferin to generate light after its activation by ATP and oxidative decarboxylation. When expressed in mammalian cells, luciferase molecules produce luminescence in direct proportion to their number in the presence of luciferin and ATP. In this respect, Luciferase is commonly used as a promoter to quantify expression levels from promoters in tissue culture and in vivo.
Catalog #L5999-27
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1-3ug/ml. Observed in mouse heart, liver, kidney, and cortex tissues containing added purified luciferase protein.
Immunohistochemistry (Frozen): 15-50ug/ml. Detects tissue specific expression of Luciferase protein in Drosophila embryos using Gal4-UAS system with two different Gal4 drivers.
Immunofluorescence: 10-30ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6A106
HostMouse
Concentration0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide
PurityPurified by Protein A chromatography
ImmunogenLuciferase protein from Photinus pyralis
SpecificityRecognizes luciferase. No cross reactivity to mouse heart, NIH3T3, Jurkat, and human fibroblasts
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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