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You are here:Home » Antibodies » Abs to Stem Cell Research » Anti -Ly-6C (Ly6C, Lymphocyte Antigen 6C2, Ly6c2, Ly-6C2, AA682074, AA959465)

Anti -Ly-6C (Ly6C, Lymphocyte Antigen 6C2, Ly6c2, Ly-6C2, AA682074, AA959465)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Purified IH FC
The antigen is a 14kD surface protein which is very similar to Ly-6C and may be analogous to human CD59. It is inducible by IFN-alpha, IFN-beta and IFN-gamma. In tissue sections, the antigen is found on macrophage precursor subpopulations. In the bone marrow and hemopoietic islands of the lymphoid organs, and in the spleen. Activated macrophages in inflammatory tissues also express the ER-MP20-related antigen.
The monoclonal antibody also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker BM8 (anti-F4/80) is recommended. In combination with an anti-mouse CD31/PECAM-1 antibody, ER-MP20 can be used to evaluate the cellular composition in murine bone marrow (e.g. using flow cytometric analysis). ER-MP20 also detects a wide range of endothelial cells.
Catalog #L7715-34G
ApplicationsSuitable for use in Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended DilutionImmunohistochemistry (frozen, paraffin): 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone NoER-MP20
HostRat
SourceMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in 0.02% sodium azide, 0.1% BSA.
PurityPurified
ImmunogenRats with mouse macrophage cell lines and reacts with mouse macrophage precursor cells.
SpecificityRecognizes specifically mouse macrophage precursor cells in the mid-stage of their development (late CFU-M, monoblasts and monocytes).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesLymphocyte Antigen 6 Complex, Locus C1, Ly6c1


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