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You are here:Home » Antibodies » Abs to Cell Surface Proteins » Anti -Lymphocyte antigen 6C2, MACROPHAGE PRECURSOR CELLS (Ly-6c)

Anti -Lymphocyte antigen 6C2, MACROPHAGE PRECURSOR CELLS (Ly-6c)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified IH FC
The antigen is a 14kD surface protein which is very similar to Ly-6C and may be analogous to human CD59. It is inducible by IFN-alpha, IFN-beta and IFN-gamma. In tissue sections, the antigen is found on macrophage precursor subpopulations. In the bone marrow and hemopoietic islands of the lymphoid organs, and in the spleen. Activated macrophages in inflammatory tissues also express the related antigen. The monoclonal antibody also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker BM8 (anti-F4/80) is recommended. In combination with an anti-mouse CD31/PECAM-1 antibody, can be used to evaluate the cellular composition in murine bone marrow (e.g. using flow cytometric analysis). Also detects a wide range of endothelial cells.
Catalog #L7715-35E
ApplicationsSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:50
Immunohistochemistry: 1:50
The antigen is glutaraldehyde (0.05%) and paraformaldehyde (1%) resistant.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No9G221
HostRat
SourceMouse
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
PurityPurified
ImmunogenMouse macrophage cell lines
SpecificityRecognizes mouse macrophage precursor cells in the mid-stage of their development (late CFU-M, monoblasts and monocytes).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesLymphocyte antigen 6C2


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