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LY294002 (PI 3 Kinase Inhibitor, 2-(4-Morpholinyl)-8-phenyl-4H-1- benzopyran-4-one)
LY294002 (PI 3 Kinase Inhibitor, 2-(4-Morpholinyl)-8-phenyl-4H-1- benzopyran-4-one)
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| LY294002 is a potent and specific cell-permeable inhibitor of phosphatidylinositol 3-kinase (PI3-kinase). LY294002 at a concentration of 50uM completely abolishes the PI3-K activity of intact neutrophils stimulated with fMet-Leu-Phe, without apparent cell toxicity. While concentration of 50uM is observed to be efficacious for inhibiting PI3-K activity in stimulated neutrophils, this concentration produces no significant inhibition of other kinases including PKC, PKA, MAP kinase, S6 kinase, EGF receptor tyrosine kinase, Src, PI4-kinase, diacylglycerol kinase, or rabbit kidney ATPase. This compound is a useful tool for identifying cellular event that are regulated by the PI3-kinase/Akt axis, and is observed to induce apoptosis in many cell types by blocking the PI3-kinase/Akt anti-apoptotic pathway. | | | Catalog # | L7751 | | LY294002 (PI3 Kinase Inhibitor) was shown to act in vivo as a highly selective inhibitor of phosphatidylinositol 3 (PI3) kinase. When used at a concentration of 50uM, it specifically abolished PI3 kinase activity (IC50=0.43ug/ml; 1.40uM) but did not inhibit other lipid and protein kinases such as PI4 kinase, PKC, MAP kinase or c-Src (1). LY294002 has been shown to block PI3 kinase-dependent Akt phosphorylation and kinase activity. | | Applications | Sutiable for use in vitro and in vivo. | | Recommended Dilutions | Cultured cells: Recommend treating the cells with LY294002 for one hour prior to, and for the duration of, the stimulation. | | Optimal dilutions to be determined by researher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Light sensitive. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Formula | C19H17NO3 | | Molecular Weight | 307.35D | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in DMSO. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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