Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Amino Acids » Anti -Lysine, Acetylated (Acetyl-Lysine)

Anti -Lysine, Acetylated (Acetyl-Lysine)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH IF
Acetylation of lysine, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (1). Signaling resulting in acetylation/deacetylation of histones, transcription factors, and other proteins affects a diverse array of cellular processes including chromatin structure and gene activity, cell growth, differentiation, and apoptosis (2-6). Recent proteomic surveys suggest that acetylation of lysine residues may be a widespread and important form of posttranslational protein modification that affects thousands of proteins involved in control of cell cycle and metabolism, longevity, actin polymerization, and nuclear transport (7,8). The regulation of protein acetylation status is impaired in cancer and polyglutamine diseases (9), and HDACs have become promising targets for anti-cancer drugs currently in development (10).
Catalog #L9058-01
ApplicationsSuitable for use in Western Blot, Immunoprecipation, Chromatin Immunoprecipitation, Immunohistcohemistry, Immunofluorescence and ELISA. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:1000
Immunoprecipitation: 1:100
Chromatin IP: 1:50
Immunohistochemistry (paraffin): 1:1600
Immunofluorescence (IF-IC): 1:200
ELISA (peptide): 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
PurityPurified by Protein A and immunoaffinity chromatography.
ImmunogenSynthetic acetylated lysine-containing peptide
SpecificityRecognizes proteins post-translationally modified by acetylation on the epsilon-amino groups of lysine residues. Recognizes acetylated lysine in a wide range of sequence contexts. It has been demonstrated to recognize acetylated histones, p53, CBP, PCAF and chemically acetylated BSA. Has been shown to react with as little as 0.04ng of chemically acetylated BSA while not recognizing up to 25ug of nonacetylated BSA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links