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Anti -Lysine, Acetylated (Acetyl-Lysine)
Anti -Lysine, Acetylated (Acetyl-Lysine)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IP |
|
| Catalog # | L9058-02A | | Applications | Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Western Blot: 0.5-2ug/ml detects acetylated histones in acid-extracted proteins from sodium butyrate (5mM) treated HeLa cells. Sodium butyrate, an inhibitor of deacetylases, was used to increase the level of acetylated histones. 0.5-4ug/ml detected: chemically acetylated BSA (10ng), GST-p53 (400ng) acetylated in vitro with either recombinant p300 or PCAF, auto-acetylated p300 (400ng) and PCAF (400ng). Proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with 4ug/ml L9058-02A. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. | | Immunoprecipitation | 5ug immunoprecipitates in vitro acetylated PCAF added to a 3T3 RIPA cell lysate. The immunoprecipitated PCAF was detected by subsequent immunoblot analysis using 1ug/ml monoclonal anti-GST. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 4H349 | | Host | Mouse | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 70% storage buffer (0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide) and 30% glycerol. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Mixture of chemically acetylated antigens | | Specificity | Recognizes acetylated lysine-containing proteins including histones. Of all the possible H3 and H4 acetylation sites, this antibody exhibits highest affinity for histone H4 acetylated on lysine 8 and histone H3 acetylated on lysine 14. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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