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Anti -Lysine, Acetylated (Acetyl-Lysine)
Anti -Lysine, Acetylated (Acetyl-Lysine)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
E B IP |
|
| Acetylation of lysine, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (1). Signaling resulting in acetylation/deacetylation of histones, transcription factors and other proteins affects a diverse array of cellular processes including chromatin structure and gene activity, cell growth, differentiation and apoptosis (2-6). Recent proteomic surveys suggest that acetylation of lysine residues may be a widespread and important form of posttranslational protein modification that affects thousands of proteins involved in control of cell cycle and metabolism, longevity, actin polymerization and nuclear transport (7,8). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (9), and HDACs have become promising targets for anti-cancer drugs currently in development (10). | | | Catalog # | L9058-02B | | Applications | Suitable for use in ELISA, Western Blot, Chromatin IP and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | ELISA (Peptide): 1:1000 | | Western Blot: 1:1000 | | Immunoprecipitation: 1:100 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 10g202 | | Host | Rabbit | | Concentration | 100ul | | (10 western blots) | | Form | Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol. | | Purity | Supernatant | | Immunogen | Synthetic acetyl-lysine peptide library. The antibody is formulated from two rabbit monoclones in order to cover broad range of reactivity. | | Specificity | Recognizes acetylated lysine in a wide range of sequence contexts. Species crossreactivity: Broad crossreactivity expected. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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