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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Lysine, Acetylated (Acetyl-Lysine)

Anti -Lysine, Acetylated (Acetyl-Lysine)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IP
Catalog #L9058-03
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunoblot Analysis: 1:1000-1:2000 dilution of this lot detected: acetylated histones in acid-extracted proteins from sodium butyrate (5mM) treated HeLa cells. Sodium butyrate, an inhibitor of deacetylases, was used to increase the level of acetylated histones. (Panel A). Chemically acetylated BSA (10ng) in a dot blot assay. A 1:1000 dilution of the previous lot of the antibody detected: acetylated histones and to a lesser degree, other proteins in RIPA lysate from trichostatin A (5uM) treated Cos-1 cells. Trichostatin A, an inhibitor of deacetylases also, was used to increase the level of acetylated histones. (Panel B). GST-p53 (100ng) acetylated in vitro with either recombinant p300 or PCAF. (Panel C)
Immunoprecipitation: 5ul of a previous lot immunoprecipitated in vitro acetylated recombinant p300.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
ConcentrationNot determined
FormSupplied as a liquid containing 0.05% sodium azide and 30% glycerol.
PuritySerum
ImmunogenA mixture of chemically acetylated antigens
SpecificityRecognizes acetyl-lysine containing proteins including histones, from sodium butyrate or Trichostatin A treated cells, p53 acetylated in vitro by p300 or PCAF, and chemically acetylated BSA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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