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Anti -Lysine, Acetylated (Acetyl-Lysine) (Biotin)
Anti -Lysine, Acetylated (Acetyl-Lysine) (Biotin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B IP IF RIA |
|
| Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine N -acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anti- cancer drugs currently in development (5). | | | Catalog # | L9058-05A | | Applications | Suitable for use in RIA, Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Western Blot:1:250 detects the acetylated histone from TSA-treated mouse spleen cells. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Concentration | ~0.25mg/ml | | Form | Supplied as a liquid in PBS, pH7, 50% glycerol. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Acetylated KLH. | | Specificity | Recognizes proteins containing acetylated lysine residues in SDS-PAGE immunoblots. Species Crossreactivity: Acetylated lysine residues. No reaction to non-acetylated proteins. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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