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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Lysine, methylated (Methyl-Lysine) (HRP)

Anti -Lysine, methylated (Methyl-Lysine) (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves methylation. Lysine can be methylated once, twice or three times by lysine methyltransferases. The transfer of methyl groups from S-adenosyl methionine to histones is catalyzed by enzymes known as histone methyltransferases. Histones which are methylated on certain residues can act epigenetically to repress or activate gene expression. The transcriptional repressor SUV39H1 can encode novel enzymes which selectively methylate histone H3 at lysine 9. SUV39H1 places a methyl marker on histone H3, which is then recognized by HP1 through its chromo domain. This model may also explain the stable inheritance of the heterochromatic state. Some studies have also speculated a stimulatory role for transcription by methylated histone lyside 4 due to its presence at active transcription sites.
Catalog #L9058-30A
ApplicationsSuitabale for use in Western Blot and ELISA. Other applications not tested.
Recommended DilutionsWestern Blot: 0.2-0.5ug/ml was sufficient for detection of the methylated histone by Western Blot analysis using melanoma cells in TBSt.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.0, 50% glycerol. Labeled with horseradish peroxidase (HRP).
PurityPurified by affinity chromatography.
ImmunogenMethylated lysine, conjugated with KLH.
SpecificityRecognizes proteins containing methylated lysine residues in SDS-PAGE immunoblots. Species Crossreactivity: multi-species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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