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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Lysine, methylated (Methyl-Lysine)

Anti -Lysine, methylated (Methyl-Lysine)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IP
Methylated lysine is found in a number of proteins such as histones, non-histone chromosomal proteins, ribosomal proteins, calmodulin, cytochrome C, etc. This evolutionarily conserved post-translational modification is an important process for many protein-protein interactions. Histone lysine methylation is a commonly studied posttranslational modification. Studies have shown the transcriptional repressor SUV39H1 can methylate lysine 9 of histone H3. It has been hypothesisized that methyl-lysine 9 provides a binding site for the chromodomain of HP1. HP1 binding is involved in the repression of euchromatic genes and is essential for heterochromatic silencing. The repressive role for transcription by methylated lysine 9 is validated by its presence at silent heterchormatic sites. Some studies have also speculated a stimulatory role for transcription by methylated histone lysine 4 due to presence at active transcription sites (1-3).
Catalog #L9058-30D
ApplicationsSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionELISA: 0.1ug/ml
Immunoprecipitation: 5-10ug/ml
Western Blot: 0.2-0.5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
ConcentrationNot determined
FormSupplied as a liquid in Tris buffer containing 50% glycerol.
PuritySerum
ImmunogenMethylated Lysine conjugated to KLH.
SpecificityRecognizes proteins methylated on lysine residues (mono, di-methylysine). Does not crossreact with acetylated protein. Species Crossreactivity: Methylated lysine residues
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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