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You are here:Home » Antibodies » Abs to Enzymes, Phosphodiesterase » Anti -Sphingomyelin phosphodiesterase 1 (Acid sphingomyelinase, ASM, aSMase)

Anti -Sphingomyelin phosphodiesterase 1 (Acid sphingomyelinase, ASM, aSMase)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
SMPD1 (Sphingomyelin phosphodiesterase 1; also ASM) is a 70-85kD member of the acid sphingomyelinase family of enzymes. It is a monomeric lysosomal enzyme that converts sphingomyelin (a plasma membrane lipid) into ceramide through the removal of phosphorylcholine. This generates second messenger components that participate in signal transduction. Mature human SMPD1 is 583aa in length. It contains one saposin B-type domain (aa85-169) that likely interacts with sphingomyelin, and a catalytic region (aa267-363). There are multiple isoforms. One is 57kD in size and results from proteolytic cleavage between Pro179-Pro197. Three others show aa substitutions; a 56 aa substitution for aa1-361, a two aa substitution for aa 363-629, and a 12aa substitution for aa363-418. Over aa62-628, human SMPD1 is 86% aa identical to mouse SMPD1.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Catalog #L9189-95B
Recommended DilutionsELISA: 0.5-1.0ug/ml. The detection limit for rhSMPD1 is ~0.5ng/well.
Western Blot: 1.0ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by affinity chromatography.
ImmunogenCHO cell-derived, recombinant human SMPD1 isoform 1.
SpecificityRecognizes human SMPD1. Species Crossreactivity: recombinant mouse SMPD1, 10%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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