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Anti -Lysosome Associated Membrane Protein 3 (LAMP 3, Dendritic Cell Lysosome Associated Membrane Protein, DC-LAMP, CD208)
Anti -Lysosome Associated Membrane Protein 3 (LAMP 3, Dendritic Cell Lysosome Associated
Membrane Protein, DC-LAMP, CD208)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B FC |
|
| DC-LAMP (dendritic cell lysosome-associated membrane protein: also known as CD208 and LAMP3) is a 50-70kD member of the LAMP family of proteins. Mature human DC-LAMP is a 389 amino acid type I transmembrane protein. It has a 254aa lumenal N-terminus and a short 14aa cytoplasmic tail. The molecule is found in type II pneumocytes, interdigitating DC, and various tumors. DC-LAMP serves as a marker of human mature DC, and it may play a role in normal lysosome and endosome function. Over the region used as immunogen, human DC-LAMP shares 72% and 55% aa sequence identity with canine and mouse DC-LAMP, respectively. | | | Catalog # | L9190-20 | | Applications | Suitable for use in ELISA, Western Blot and Flow Cytometry. Other applications have not been tested. | | Recommended Dilution | Western Blot: 0.1-0.2ug/ml Detection limit is ~0.5 ng/lane under non-reducing and reducing conditions. | | Direct ELISA: 0.5-1.0ug/ml Detection limit is ~0.1 ng/well. | | Flow Cytometry: 25ug/ml. For monocyte-derived mature dendritic cells, use 10ul of diluted solution for 1-2.5x10e5 cells in total volume 200ul. | | Optimal dilutions to be determined by researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | ~0.2mg/ml | | Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Recombinant corresponding to aa21-381 of human DC-LAMP lumenal domain expressed in NS0 cells (EAW78337). Species sequence homology: canine 72%, mouse 55%. | | Specificity | Recognizes human LAMP3. In ELISA, displays less than 1% cross-reactivity with rhLAMP. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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