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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IP

Lysozyme is a 14kD enzyme directed against the b1 a 4 glycosidic bond between N-acetylglucosamine and Nacetylmuramic acid residues that make up peptidoglycan. Lysozyme is an antimicrobial protein secreted by polymorphonuclear leukocytes and is widely distributed in secretions such as airway secretions and nasal fluid whereas it is the most effective antimicrobial protein. It is also produced by monocytes, macrophages and epithelial cells. Lysozyme is able to kill bacteria by enzymatic lysis of bacterial cell walls and by a nonenzymatic mechanism.
Catalog #	L9200-05D
	Allthough lysozyme is highly active against many gram-positive bacteria it is ineffective against gram-negative bacteria unless potentiated by certain cofactors (lactoferrin, antibody-complement or hydrogen peroxide-ascorbic acid). Next to its antimicrobial activity lysozyme has many other physiological functions including inactivation of certain viruses, important roles in surveillance of membranes of mammalian cells, immune regulatory activity, antiinflammatory and antitumor activity
Applications	Suitable for use in Western Blot, Immunoassays, and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Western Blot: 1:10
	Immunoprecipitation: 1:10
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	As reported
Form	Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Purity	Purified
Immunogen	Lysozyme.
Specificity	Recognizes human lysozyme.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.