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L9200-05D Rabbit Anti-Lysozyme

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
IP WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Lysozyme is a 14kD enzyme directed against the b1 a 4 glycosidic bond between N-acetylglucosamine and Nacetylmuramic acid residues that make up peptidoglycan. Lysozyme is an antimicrobial protein secreted by polymorphonuclear leukocytes and is widely distributed in secretions such as airway secretions and nasal fluid whereas it is the most effective antimicrobial protein. It is also produced by monocytes, macrophages and epithelial cells. Lysozyme is able to kill bacteria by enzymatic lysis of bacterial cell walls and by a nonenzymatic mechanism. Allthough lysozyme is highly active against many gram-positive bacteria it is ineffective against gram-negative bacteria unless potentiated by certain cofactors (lactoferrin, antibody-complement or hydrogen peroxide-ascorbic acid). Next to its antimicrobial activity lysozyme has many other physiological functions including inactivation of certain viruses, important roles in surveillance of membranes of mammalian cells, immune regulatory activity, antiinflammatory and antitumor activity

Applications
Suitable for use in Western Blot, ELISA and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Western Blot: 1:10 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human lysozyme.
References
1. Laible, N et al; Bactericidal activity of human lysozyme, muramidase inactivating lysozyme, and cationic polypeptides against Streptococcus sanguis and Streptococcus faecalis: inhibition by chitin oligosaccharides. Infect Immun 1985, 48: 720 2. Cole, A et al; Innate antimicrobial activity of nasal secretions. Infect Immun 1999, 67: 3267 3. Ibrahim, H et al; Strategies for new antimicrobial proteins and peptides: lysozyme and aprotinin as model molecules. Curr Pharm Des 2002, 8: 671 4. Cole, A et al; Cationic polypeptides are required for antibacterial activity of human airway fluid. J Immunol 2002, 169: 6985. 5. Dan L., et al. Expression of recombinant human lysozyme in bacterial artificial chromosome transgenic mice promotes the growth of Bifidobacterium and inhibits the growth of Salmonella in the intestine. 2018. J Biotechnol. 272-272:33-39. doi: 10.1016/j.jbiotec.2018.03.005. [Epub ahead of print].
USBio References
1. Dan L., et al. Expression of recombinant human lysozyme in bacterial artificial chromosome transgenic mice promotes the growth of Bifidobacterium and inhibits the growth of Salmonella in the intestine. 2018. J Biotechnol. 272-272:33-39. doi: 10.1016/j.jbiotec.2018.03.005. [Epub ahead of print].
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