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You are here:Home » Antibodies » Abs to Enzymes, Glycosidase » Anti -Lysozyme (Muramidase)

Anti -Lysozyme (Muramidase)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IP
Lysozyme is a relatively small (129aa) secretory enzyme that catalyzes the hydrolysis of b-1,4 glucosidic linkages between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) comprising the cell walls of bacteria and to a lesser degree chitin oligomers. Lysozyme is common in animals and plants. In birds, lysozyme is also an exceptionally abundant protein in egg whites. Its biological function in fowl eggs is unclear. Hen egg white lysozyme "c" is the most commonly studied form and source of the enzyme. Lysozyme from domestic goose is designated lysozyme "g". Lysozyme is also found in vertebrates, including human, mostly in secretions and certain tissues, such as saliva, tears, milk, cervical mucus, leucocytes, kidneys and urine, where it probably serves as an anti-bacterial agent by digesting and weakening the rigid bacterial cell wall, thereby rendering the bacterial susceptible to osmotic lysis. Plant lysozyme is found in ficus and papaya latex, and chemically is distinct from the egg white enzyme.
Catalog #L9200-09
ApplicationSuitable for use in Western Blot, ELISA and Immunoprecipitation. Other applications not tested.
Recommended DilutionsELISA: 1:5000-1:20,000. 1ug of lysozyme was used in a standard sandwich ELISA using I1904-46Q (IgG, H&L (HRP)) and ABTS (A0030) as a substrate for 30 minutes at RT.
Western Blot: 1:500-1:5000
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceChicken
ConcentrationNot Determined
FormSupplied as a lyophilized powder from PBS, pH 7.2, 0.01% sodium azide. Reconstitute with 2ml sterile ddH2O.
PuritySerum
ImmunogenLysozyme from hen egg white
SpecificityRecognizes Lysozyme. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Lysozyme (hen egg white).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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