| Lysozyme is a relatively small (129aa) secretory enzyme that catalyzes the hydrolysis of b-1,4 glucosidic linkages between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) comprising the cell walls of bacteria and to a lesser degree chitin oligomers. Lysozyme is common in animals and plants. In birds, lysozyme is also an exceptionally abundant protein in egg whites. Its biological function in fowl eggs is unclear. Hen egg white lysozyme "c" is the most commonly studied form and source of the enzyme. Lysozyme from domestic goose is designated lysozyme "g". Lysozyme is also found in vertebrates, including human, mostly in secretions and certain tissues, such as saliva, tears, milk, cervical mucus, leucocytes, kidneys and urine, where it probably serves as an anti-bacterial agent by digesting and weakening the rigid bacterial cell wall, thereby rendering the bacterial susceptible to osmotic lysis. Plant lysozyme is found in ficus and papaya latex, and chemically is distinct from the egg white enzyme. |
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| Applications: |
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| Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested. |
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| Catalog # | L9200-11 |
| Recommended Dilutions | ELISA: 1:10,000 |
| Western Blot: 1:100-1:5000 |
| Immunohistochemistry: 1:500-1:2500 |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-46Q: IgG, H&L (HRP) Pab Gt xRb |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light. |
| CAS Number | N/A |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Chicken |
| Concentration | ~10mg/ml (after reconstitution) |
| Form | Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA |
| (IgG and Protease free), 0.01% gentamycin sulfate. Labeled with Horseradish Peroxidase (HRP). Reconstitute with 2ml sterile ddH2O. |
| Purity | Purified by delipidation, salt fractionation and Ion Exchange chromatography. |
| Immunogen | Lysozyme from hen egg white. |
| Specificity | Recognizes chicken Lysozyme. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum, purified and partially purified Lysozyme (hen egg white). Crossreactivity against lysozyme from other tissues and species may occur but have not been specifically determined. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
