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You are here:Home » Antibodies » Abs to Enzymes, Glycosidase » Anti -Lysozyme (Biotin) (Muramidase)

Anti -Lysozyme (Biotin) (Muramidase)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B IP IH
Lysozyme is a relatively small (129aa) secretory enzyme that catalyzes the hydrolysis of b-1,4 glucosidic linkages between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) comprising the cell walls of bacteria and to a lesser degree chitin oligomers. Lysozyme is common in animals and plants. In birds, lysozyme is also an exceptionally abundant protein in egg whites. Its biological function in fowl eggs is unclear. Hen egg white lysozyme "c" is the most commonly studied form and source of the enzyme. Lysozyme from domestic goose is designated lysozyme "g". Lysozyme is also found in vertebrates, including human, mostly in secretions and certain tissues, such as saliva, tears, milk, cervical mucus, leucocytes, kidneys and urine, where it probably serves as an anti-bacterial agent by digesting and weakening the rigid bacterial cell wall, thereby rendering the bacterial susceptible to osmotic lysis. Plant lysozyme is found in ficus and papaya latex, and chemically is distinct from the egg white enzyme.
Catalog #L9200-20A
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, ELISA and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1:2000-1:10,000
Immunoprecipitation: 1:100
ELISA: 1:20,000
Immunohistochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceChicken
Concentration~10mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide, 40% glycerol. Labeled with Biotin.
PurityPuri ed from monospeci c antiserum by delipidation, fractionation and ion exchange chromatography.
ImmunogenLysozyme [Hen Egg White]
SpecificityRecognizes Lysozyme (hen egg white). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, as well as purified and partially purified lysozyme (hen egg white). Crossreactivity against Lysozyme from other sources is unknown.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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