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You are here:Home » Culture Media » Media-Microbiological » MacConkey Agar w/Sorbitol (Powder)

MacConkey Agar w/Sorbitol (Powder)

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Specifications

Agar medium intended for the isolation and detection of Escherichia coli 0157 H7.
Escherichia coli serotype 0157:H7 was implicated in causing hemorrhagic colitis. Farmer and Davis noted that 95% of E. coli strains ferment sorbitol while almost all strains of E coli 0157:H7 did not ferment sorbitol They used this medium in their studies to estimate the number of sorbitol-negative E. coli present in stool specimens. Sorbitol fermenting bacteria appear as reddish-pink colonies and non-sorbitol fermenting bacteria appear as colorless or transparent colonies.
Catalog #M1100-35
AppearanceLight pinkish-beige, homogeneous, free-flowing powder.
pH7.1 ± 0.2
Microbiological TestsThe medium was prepared according to formulation and inoculated with the organisms listed below.
Escherichia coli ATCC 25922Growth, pink colonies (Sorbitol positive)
Escherichia coli 0157H7 ATCC 35510:
Growth, colorless colonies (Sorbitol negative)
Enterococcus faecalis ATCC 29212Inhibited (No sorbitol reaction (N/A))
Klebsiella pneumoniae ATCC 13883Growth, pink mucoid colonies (Sorbitol positive)
Proteus mirabilis ATCC 12453Colorless colonies, swarming inhibited (Sorbitol negative)
Pseudomonas aeruginosa ATCC 27853Growth, colorless colonies (Sorbitol negative)
Staphylcoccus aureus ATCC 25923Inhibited (No sorbitol reaction (N/A))
Storage and StabilityStore the sealed bottle containing the dehydrated medium at 2-30°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light pinkish-beige color.
Final pH7.1 ± 0.2 at 25.0°C
Appearance
Light pinkish-beige, homogeneous, free-flowing powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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