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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant IH

Macrophages and histiocytes, which are widely distributed throughout the body, play a crucial role in the body defense mechanisms. They are heterogenous in morphology, cytochemistry, function and surface antigens. Immunohistochemical labeling with monoclonal antibodies is one of the most specific and reliable techniques for the identification and localization of these cells. Monoclonal antibodies to macrophage and histiocyte surface or cytoplasmic antigens are important for the immunodiagnosis of both benign and malignant histiocytic lesions. They are useful in the investigation of macrophage differentiation and maturation and in the diagnosis of various inflammatory, reactive and neoplastic disorders of macrophages.
Catalog #	M1199-75C
Applications	Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Immunohistochemistry (Frozen/ Paraffin): 1:10-1:100
	Requires antigen retrieval using heat treatment prior to staining paraffin sections.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	9L463
Host	Mouse
Source	Human
Concentration	Not Determined
Form	Supplied as a liquid, 0.7% BSA, 0.09% sodium azide.
Purity	Supernatant
Immunogen	Human spleen cell homogenate, depleted of lymphocytes and erythrocytes.
Specificity	Recognizes an antigen expressed by human macrophages, monocytes and histiocytes. Does not cross-react with interdigitating dendritic cells, Langerhans cells, microglial cells or lymphoid cells. Some staining of basophilic granulocytes and of mast cells may be seen. In immunohistochemical studies the antibody shows partial cross-blocking with some anti-CD68 monoclonal antibodies.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.