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Macrophage Colony Stimulating Factor, Recombinant, Mouse (M-CSF) (formerly M2748B)
Macrophage Colony Stimulating Factor, Recombinant, Mouse (M-CSF) (formerly M2748B)
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| Macrophage Colony Stimulating Factor (M-CSF) is produced by various cell types including broblasts, macrophages and endothelial cells. This M-CSF is involved in both the development and function of monocytes and macrophages. Recombinant Murine M-CSF is a disul de-linked homodimer composed of 18.2kD subunits of 156 amino acid residues. | | | Catalog # | M1201-03 | | Biological Activity | The ED50, as determined by the dose-dependent stimulation of the proliferation of M-NFS-60 cells, is 1ng/ml, corresponding to a speci c activity of 1x10e6 units/mg. | | Amino Acid Sequence | MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Country of Origin | USA | | Source | E. coli | | Purity | 98% by SDS-PAGE and HPLC analysis. Endotoxin: 0.1ng/ug (1EU/ug). | | Form | Supplied as a lyophilized powder from 5mM Tris, pH 8.0, 100mM sodium chloride. Reconstitute with sterile dH2O to a concentration of 0.1-0.5mg/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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