| Macrophage colony stimulating factor (M-CSF) is one of the glycoproteins called colony-stimulating factors (CSFs).A mononuclear phagocyte colony-stimulating factor (M-CSF) synthesized by mesenchymal cells. The compound stimulates the survival, proliferation, and differentiation of hematopoietic cells of the monocyte-macrophage series. Some clinical investigations have shown autologous production of M-CSF various human cell lines in vitro and by tumors in vivo |
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| Catalog # | M1201-03A |
| Description | Recombinant Mouse M-CSF produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 156 amino acids and having a total molecular mass of 36.4kD. Recombinant M-CSF is purified by proprietary chromatographic techniques. |
| Amino Acid Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Lys-Glu-Val-Ser. |
| Dimers and Aggregates | Less than 1% as determined by silver-stained SDS-PAGE gel. |
| Biological Activity | Mouse M-CSF is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent stimulation of the proliferation of murine M-NFS-60 indicator cells was found < 1ng/ml, corresponding to a Specific Activity of 1 x 10e6 IU/mg. |
| Endotoxin | 0.1ng/ug (IEU/ug) of Recombinant Murine M-CSF. |
| Protein Content | Protein quantitation was carried out by two independent methods: |
| 1. UV spectroscopy at 280 nm. |
| 2. Analysis by RP-HPLC, using a calibrated solution of M-CSF as a Reference Standard. |
| Solubility | Reconstitute the lyophilized mouse M-CSF in sterile dH2O to 100ug/ml, which can then be further diluted with other aqueous solutions. |
| Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Molecular Weight | 36.4kD |
| Source | E. coli |
| Purity | 98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel. |
| Concentration | ~1mg/ml |
| Form | Supplied as a lyophilized powder in 5mM Tris, pH 8.0, 100mM sodium chloride. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
