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You are here:Home » Molecular Biology » Growth Factors-M-CSF » Macrophage Colony Stimulating Factor, Recombinant, Mouse (M-CSF)

Macrophage Colony Stimulating Factor, Recombinant, Mouse (M-CSF)

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Specifications

Macrophage colony stimulating factor (M-CSF) is one of the glycoproteins called colony-stimulating factors (CSFs).A mononuclear phagocyte colony-stimulating factor (M-CSF) synthesized by mesenchymal cells. The compound stimulates the survival, proliferation, and differentiation of hematopoietic cells of the monocyte-macrophage series. Some clinical investigations have shown autologous production of M-CSF various human cell lines in vitro and by tumors in vivo
Catalog #M1201-03A
DescriptionRecombinant Mouse M-CSF produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 156 amino acids and having a total molecular mass of 36.4kD. Recombinant M-CSF is purified by proprietary chromatographic techniques.
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Lys-Glu-Val-Ser.
Dimers and AggregatesLess than 1% as determined by silver-stained SDS-PAGE gel.
Biological ActivityMouse M-CSF is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent stimulation of the proliferation of murine M-NFS-60 indicator cells was found < 1ng/ml, corresponding to a Specific Activity of 1 x 10e6 IU/mg.
Endotoxin 0.1ng/ug (IEU/ug) of Recombinant Murine M-CSF.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a calibrated solution of M-CSF as a Reference Standard.
SolubilityReconstitute the lyophilized mouse M-CSF in sterile dH2O to 100ug/ml, which can then be further diluted with other aqueous solutions.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight36.4kD
SourceE. coli
Purity98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Concentration~1mg/ml
FormSupplied as a lyophilized powder in 5mM Tris, pH 8.0, 100mM sodium chloride.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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