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You are here:Home » Molecular Biology » Growth Factors-M-CSF » Macrophage Colony Stimulating Factor, Recombinant, Mouse (M-CSF)

Macrophage Colony Stimulating Factor, Recombinant, Mouse (M-CSF)

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Specifications

M-CSF is produced by a wide variety of cells including fibroblasts, monocytes, lymphocytes, osteoblasts, and mesenchymal cells to name a few. It stimulates the proliferation and differentiation of hematopoietic cells and plays an important role in the life of blood monocytes. Comprised of two 159 amino acid chains, this disulfide-linked homodimer has a molecular weight of 36.8kD.
Catalog #M1201-03B
Recombinant protein corresponding to mouse Macrophage Colony Stimulating Factor expressed in E. coli.
Biological ActivityThe activity is determined by the dose-dependent induction of M-NFS-60 cell proliferation and is typically 1.1-1.6ng/ml.
AA SequenceMKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight36.8kD
SourceE. coli
Purity98% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE.. Endotoxin: 0.1EU/ug
FormSupplied as a lyophilized powder, pH 8.0. Reconstitute with sterile ddH2O, 0.1% BSA or HSA to 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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