Macrophage Colony Stimulating Factor, Recombinant, Human (M-CSF, CSF-1, Lanimostim,
Macrophage colony-stimulating factor 1 precursor, MCSF, M-CSF, MGC31930)
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| Macrophage colony stimulating factor (M-CSF) is one of the glycoproteins called colony-stimulating factors (CSFs).A mononuclear phagocyte colony-stimulating factor (M-CSF) synthesized by mesenchymal cells. The compound stimulates the survival, proliferation, and differentiation of hematopoietic cells of the monocyte-macrophage series. Some clinical investigations have shown autologous production of M-CSF various human cell lines in vitro and by tumors in vivo. | | | Catalog # | M1201A | | Recombinant Human M-CSF is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 159 amino acids and having a total molecular mass of 36.8kD. | | Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Glu-Val-Ser. | | Biological Activity | M-CSF is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent stimulation of the proliferation of murine M-NFS-60 indicator cells was found < 5ng/ml, corresponding to a Specific Activity of 2 x 10e5 IU/mg. | | Endotoxin | Less than 0.1ng/ug (IEU/ug) of M-CSF. | | Storage and Stability | Store powder dessicated at -20ºC. Liquid can be stored at 4ºC for 2-7 days, long term at -20ºC. For long term it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to prevent repeated freeze-thaw cycles. | | | | Source | Recombinant, Human (E. coli) | | Purity | 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: 0.1ng/ug (IEU/ug) | | Form | Supplied as a lyophilized powder in HSA, Manntiol. Reconstitute sterile ddH2O, 0.1% HSA or BSA to 100ug/ml, which can then be further diluted to other aqueous solutions | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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