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Macrophage Colony Stimulating Factor, Recombinant, Human (M-CSF, CSF-1, Lanimostim, Macrophage colony-stimulating factor 1 precursor, MCSF, M-CSF, MGC31930)
Macrophage Colony Stimulating Factor, Recombinant, Human (M-CSF, CSF-1, Lanimostim,
Macrophage colony-stimulating factor 1 precursor, MCSF, M-CSF, MGC31930)
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| Macrophage colony stimulating factor (M-CSF) is produced by many cell types, including fibroblasts, endothelial cells, monocytes and granulocytes. M-CSF was initially identified on the basis of its ability to stimulate the growth of macrophage/granulocyte containing colonies in soft agar cultures. M-CSF stimulates the development of hemopoietic stem cells into macrophages and supports the growth and differentiation of monocytes. | | | Catalog # | M1201B | | Source | Recombinant corresponding to 158aa from human Macrophage Colony Stimulating Factor expressed in E. coli. | | Molecular Weight | ~18.4kD | | Endotoxin | ~0.1ng/ug | | Biological Activity | ED50 1.0ng/ml ( 1x10e6units/mg). The biological activity was determined by measuring the dose dependent stimulation of proliferation of mouse M-NSF-60 cells. The optimal concentration should be determined for each specific application. | | Recommended Dilutions | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months. | | Molecular Weight | 36.8kD | | Source | Recombinant, Human (E. coli) | | Purity | ~98% (SDS PAGE, HPLC) | | Concentration | Not determined | | Form | Supplied as a lyophilized powder from 5mM Tris, pH 8.0, with 75mM sodium chloride. Reconstitute with sterile dH2O to 0.1-0.5mg/ml. Do not vortex. Further dilutions should be made in aqueous buffers supplemented with carrier protein, such as 0.1% BSA. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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