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You are here:Home » Molecular Biology » Growth Factors-MIP » Macrophage Inflammatory Protein 1 alpha, Recombinant, Rat (MIP-1a, CCL3)

Macrophage Inflammatory Protein 1 alpha, Recombinant, Rat (MIP-1a, CCL3)

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Specifications

Recombinant Rat MIP-1a produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7853 Dalton. Recombinant Rat MIP-1a is purified by proprietary chromatographic techniques.
Catalog #M1202-10C
Amino Acid SequenceThe sequence of the first five N-terminal amino acids: Ala-Pro-Tyr-Gly-Ala.
Biological ActivityRecombinant Rat MIP-1a is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract of rat peritoneal macrophages using a conc. of 60 ng/ml.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.38 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of MIP-1 alpha as a Reference Standard.
ReconsitutionReconstitute in ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized MIP-1alpha although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution MIP-1 alpha should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Molecular Weight7.85kD
SourceE. coli
Purity95% as determined by RP-HPLC, or SDS-PAGE.
Concentration1mg/ml
FormSupplied as a lyophilized powder without additives.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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