CCL20, also known as macrophage inflammatory protein 3 alpha (MIP-3a), is a b-chemokine that is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. CCL20 binds to CCR6 to promote chemotaxis of lymphocytes and inhibit the proliferation of myeloid progenitors.
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Purified recombinant protein corresponding Ala27-Met96 (Asn95Asp) from human Macrophage Inflammatory Protein 3 alpha, expressed in E. coli.
Form
Supplied as a liquid in PBS. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein G affinity chromatography from ascites.|Endotoxin: <0.10EU/ug (LAL)
Specificity
Recognizes human Macrophage Inflammatory Protein 3 alpha. Does not cross-react with recombinant human CCL19, CXCL1, CCL16, CCL11, recombinant mouse CCL20, or recombinant rat CCL20.
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