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Macrophage Inflammatory Protein 3 beta, Recombinant, Human (MIP-3b, Exodus-3, ELC)
Macrophage Inflammatory Protein 3 beta, Recombinant, Human (MIP-3b, Exodus-3, ELC)
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| MIP-3 beta is a CC chemokine that is expressed in the thymus, lymph nodes and in activated bone marrow stromal cells and signals through the CCR7 receptor. MIP-3 beta is a chemoattractant for T and B lymphocytes and myeloid progenitor cells. Human MIP-3 beta is active on murine cells. Recombinant human MIP-3 beta is an 8.8kD protein containing 77 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines. | | | Catalog # | M1202-65 | | AA Sequence | GTNDAEDCCL SVTQKPIPGY IVRNFHYLLI KDGCRVPAVV FTTLRGRQLC APPDQPWVER IIQRLQRTSA KMKRRSS | | Reconstitution | Reconstitute with sterile dH2O to 0.1-1.0mg/ml. This solution can then be diluted into other buffers and stored at 4°C for 1 week or stored at -20°C for future use. | | Biological Activity | Determined by its ability to chemoattract human T cells using a concentration of 10-50ng/ml. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 8.8kD | | Source | E. coli | | Purity | 98% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1ng/ug (1EU/ug) | | Concentration | As reported | | Form | Supplied as a lyophilized powder from a sterile filtered solution, lyophilized without additives. Reconstitute with sterile dH2O to 0.1-1.0mg/ml. This solution can then be diluted into other buffers. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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