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You are here:Home » Molecular Biology » Growth Factors-MIP » Macrophage Inflammatory Protein 3 beta, Recombinant, Mouse (MIP-3b, Exodus-3, ELC)

Macrophage Inflammatory Protein 3 beta, Recombinant, Mouse (MIP-3b, Exodus-3, ELC)

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Specifications

Recombinant Mouse MIP-3beta produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 83 amino acids and having a molecular mass of 9216 Dalton. Recombinant MIP-3b is purified by proprietary chromatographic techniques.
Catalog #M1202-65F
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be, Gly-Ala-Asn-Asp-Ala.
Dimers and AggregatesLess than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityRecombinant MIP-3 beta is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract Human mature dendritic cells using a concentration of 10-100 ng/ml.
Endotoxin 0.1ng/ug (IEU/ug) of MIP-3b.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.91 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of MIP-3beta as a Reference Standard.
SolubilityIt is recommended to reconstitute the lyophilized Mouse MIP-3 b in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized MIP-3beta although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution murine MIP-3b should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Molecular Weight9.22kD
SourceE. coli
Purity95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Concentration~1mg/ml
FormSupplied as a lyophilized powder without additives.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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