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Macrophage Inflammatory Protein 3 beta, Recombinant, Mouse (MIP-3b, Exodus-3, ELC)
Macrophage Inflammatory Protein 3 beta, Recombinant, Mouse (MIP-3b, Exodus-3, ELC)
Pricing
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| Recombinant Mouse MIP-3beta produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 83 amino acids and having a molecular mass of 9216 Dalton. Recombinant MIP-3b is purified by proprietary chromatographic techniques. | | | Catalog # | M1202-65F | | Amino Acid Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be, Gly-Ala-Asn-Asp-Ala. | | Dimers and Aggregates | Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | | Biological Activity | Recombinant MIP-3 beta is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract Human mature dendritic cells using a concentration of 10-100 ng/ml. | | Endotoxin | 0.1ng/ug (IEU/ug) of MIP-3b. | | Protein Content | Protein quantitation was carried out by two independent methods: | | 1. UV spectroscopy at 280 nm using the absorbency value of 0.91 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). | | 2. Analysis by RP-HPLC, using a calibrated solution of MIP-3beta as a Reference Standard. | | Solubility | It is recommended to reconstitute the lyophilized Mouse MIP-3 b in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | | Stability | Lyophilized MIP-3beta although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution murine MIP-3b should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. | | Molecular Weight | 9.22kD | | Source | E. coli | | Purity | 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel. | | Concentration | ~1mg/ml | | Form | Supplied as a lyophilized powder without additives. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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