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You are here:Home » Molecular Biology » Growth Factors-MIP » Macrophage Inflammatory Protein 4, Recombinant, Human (MIP-4, CCL18)

Macrophage Inflammatory Protein 4, Recombinant, Human (MIP-4, CCL18)

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Specifications

Recombinant Human Macrophage Inflammatory Protein-4 is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7813 Daltons.
Catalog #M1202-70A
SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Ala-Gln-Val-Gly-Thr.
Dimers and Aggregates 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityMurine Macrophage Inflammatory Protein-4 is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract Human T lymphocytes at 1.0-10.0ng/ml.
Endotoxin 0.1ng/ug (IEU/ug) of Recombinant Human MIP-4.
Protein ContentMIP-4 protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a calibrated solution of Recombinant Human MIP-4 as a Reference Standard.
ReconstitutionReconstitute the lyophilized MIP-4 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
SourceE. coli
Purity98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder. No additives.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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