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You are here:Home » Antibodies » Abs to Disease Markers » Anti -Macrophage (L1 Protein, Calprotectin, Cystic Fibrosis Antigen)

Anti -Macrophage (L1 Protein, Calprotectin, Cystic Fibrosis Antigen)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC
The L1 or Calprotectin molecule is an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia.
Catalog #M1202-76
ApplicationsSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:50-1:100; 10ul lables 1x10e6 cells in 100ul. Membrane permeabilization is required for this application.
Immunohistochemistry (Frozen and Paraffin): 1:100-1:200; Prior to staining paraffin sections, product requires protein digestion pre-treatment e.g. trypsin, 0.1% for 10 minutes or antigen retrieval using heat treatment. Frozen sections do not require pretreatment.
Optimal working dilutions to be determined by researcher.
Recommended Positive ControlHuman spleen
Recommended Negative ControlI1904-78R: IgG1: Murine Negative Control
Recommended Secondary AntibodiesI1904-65W: IgG, F(abí)2 (HRP) Pab Rb x Mo
I1904-66C: IgG, F(abí)2 X-adsorbed (HRP) Pab Rb x Mo
I1904-66A: IgG, F(abí)2 (FITC) Pab Rb x Mo
HybridomaNS1 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H2617
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman monocytes
SpecificityRecognizes human L1 or Calprotectin molecule. Species Crossreactivity: Rhesus and Cynomolgus monkey, baboon, feline, canine, guinea pig, equine, rabbit, rat, goat, porcine, bovine and Fallow deer.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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