| The L1 or Calprotectin molecule is an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. |
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| Catalog # | M1202-76 |
| Applications | Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. |
| Recommended Dilutions | Flow Cytometry: 1:50-1:100; Membrane permeabilization is required for this application. |
| Immunohistochemistry (Frozen and Paraffin): 1:100-1:200; Prior to staining paraffin sections, product requires protein digestion pre-treatment e.g. trypsin, 0.1% for 10 minutes or antigen retrieval using heat treatment. Frozen sections do not require pretreatment. |
| Optimal working dilutions to be determined by researcher. |
| Recommended Positive Control | Human spleen |
| Recommended Negative Control | I1904-78R: IgG1: Murine Negative Control |
| Recommended Secondary Antibodies | I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb x Mo |
| I1904-66C: IgG, F(ab’)2 X-adsorbed (HRP) Pab Rb x Mo |
| I1904-66A: IgG, F(ab’)2 (FITC) Pab Rb x Mo |
| Hybridoma | NS1 myeloma cells with spleen cells from Balb/c mice. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H2617 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, 0.09% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Human monocytes |
| Specificity | Recognizes human L1 or Calprotectin molecule. Species Crossreactivity: Rhesus and Cynomolgus monkey, baboon, feline, canine, guinea pig, equine, rabbit, rat, goat, porcine and bovine. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
