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You are here:Home » Antibodies » Abs to Cells, Blood Components » Anti -Macrophage

Anti -Macrophage

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC
Macrophages and histiocytes, which are widely distributed throughout the body, play a crucial role in the body defense mechanisms. They are heterogenous in morphology, cytochemistry, function and surface antigens. Immunohistochemical labeling with monoclonal antibodies is one of the most specific and reliable techniques for the identification and localization of these cells. Monoclonal antibodies to macrophage and histiocyte surface or cytoplasmic antigens are important for the immunodiagnosis of both benign and malignant histiocytic lesions. They are useful in the investigation of macrophage differentiation and maturation and in the diagnosis of various inflammatory, reactive and neoplastic disorders of macrophages.
Catalog #M1202-77
ApplicationsSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:10
Immunohistochemistry (Frozen): 1:100
Optimal dilutions to be determined by the researcher.
HybridomaDerived from hybridization of mouse SP2/0 myeloma cells with spleen cells from Balb/c mice immunized with WAG/Rij lymphnode cells.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No6A592
HostMouse
SourceRat
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.01% sodium azide, 50% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenWAG/Rij lymphnode cells
SpecificityRecognizes rat macrophages and is reactive with all common inbred strains.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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