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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IH FC

Macrophages and histiocytes, which are widely distributed throughout the body, play a crucial role in the body defense mechanisms. They are heterogenous in morphology, cytochemistry, function and surface antigens. Immunohistochemical labeling with monoclonal antibodies is one of the most specific and reliable techniques for the identification and localization of these cells. Monoclonal antibodies to macrophage and histiocyte surface or cytoplasmic antigens are important for the immunodiagnosis of both benign and malignant histiocytic lesions. They are useful in the investigation of macrophage differentiation and maturation and in the diagnosis of various inflammatory, reactive and neoplastic disorders of macrophages.
Catalog #	M1202-77
Applications	Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Flow Cytometry: 1:10
	Immunohistochemistry (Frozen): 1:100
	Optimal dilutions to be determined by the researcher.
Hybridoma	Derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from Balb/c mice immunized with WAG/Rij lymphnode cells.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	6A592
Host	Mouse
Source	Rat
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, 0.01% sodium azide, 50% glycerol.
Purity	Purified by Protein G affinity chromatography.
Immunogen	WAG/Rij lymphnode cells
Specificity	Recognizes rat macrophages and is reactive with all common inbred strains.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.