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Macrophage Inflammatory Protein 1 alpha, Recombinant, Human (MIP-1 alpha, CCL3)
Macrophage Inflammatory Protein 1 alpha, Recombinant, Human (MIP-1 alpha, CCL3)
Pricing
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| Recombinant Human MIP-1 is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7820 Dalton. | | | Catalog # | M1202A | | Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be, Ala-Ser-Leu-Ala-Ala. | | Dimers and Aggregates | 1% as determined by silver-stained SDS-PAGE gel analysis. | | Biological Activity | UV spectroscopy at 280nm using the absorbency value of 0.9 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). | | Endotoxin | 0.1ng/ug (IEU/ug) of rHuMIP-1. | | Protein Content | Protein quantitation was carried out by two independent methods: | | 1. UV spectroscopy at 280nm. | | 2. Analysis by RP-HPLC, using a standard solution of MIP-1 as a Reference Standard. | | Reconstitution | Reconstitute the lyophilized rHuMIP-1 in sterile 18M -cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. | | Source | E. coli | | Purity | 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. | | Concentration | ~0.1mg/ml | | Form | Supplied as a lyophilized powder. No additives. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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