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You are here:Home » Molecular Biology » Growth Factors-MIP » Macrophage Inflammatory Protein-1 alpha, Recombinant, Human (MIP-1-alpha, MIP1A, C-C Motif Chemokine 3, Chemokine (C-C motif) Ligand 3, CCL3, G0/G1 Switch Regulatory Protein 19-1, G0S19-1, LD78alpha, PAT 464.1, SIS-beta, Small-inducible Cytokine A3, SCYA3

Macrophage Inflammatory Protein-1 alpha, Recombinant, Human (MIP-1-alpha, MIP1A, C-C Motif
Chemokine 3, Chemokine (C-C motif) Ligand 3, CCL3, G0/G1 Switch Regulatory Protein 19-1,
G0S19-1, LD78alpha, PAT 464.1, SIS-beta, Small-inducible Cytokine A3, SCYA3

Pricing

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Specifications

Catalog #M1202B
SourceRecombinant corresponding to 70aa of human Macrophage Inflammatory Protein-1 alpha expressed in E.coli is a single, non-glycosylated,polypeptide chain.
Molecular Weight~7.8kD
Biological ActivityUV spectroscopy at 280nm using the absorbency value of 0.9 as the extinctioncoefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics).
Dimers and Aggregates 1% (SDS-PAGE)
Protein ContentProtein quantitation was carried out by two independent methods: 1.UV spectroscopy at 280nm . 2. Analysis by RP-HPLC, using a standard solution of MIP-1 as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~7.8kD
Purity~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
Concentration~0.55mg/ml
FormSupplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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