Anti -Macrophage, Monocyte Chemotactic Protein-1 (MCP-1, Chemokine C-C Motif Ligand 2,
CCL2, GDCF-2, HC11, HSMCR30, JE, MGC9434, Monocyte Chemoattractant Protein 1, Monocyte
Chemotactic and Activating Factor, Monocyte Chemotactic Protein 1, Monocyte Secretory P
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IH |
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| Rat MCP-1/JE (monocyte chemoattracctant protein-1) is a 148-amino acid CC chemokine with a NH2-terminal sequence of 29 residues as a signal sequence. It was originally cloned from Con A-stimulated rat spleen cDNA library (1). This rat MCP-1/JE is 49-amino acid longer than human MCP-1 at 3’-end. This 3’-end is a serine and threonine rich zone, which is probably responsible for the extensive O-glycosylation and explains for the higher molecular weight (25kD) (2). In vitro, MCP-1/JE is chemotactic for monocytes as well as lymphocytes and basophils, but not for neutrophils. MCP-1/JE is produced by a wide range of cell types as a reaction to diverse inflammatory stimuli (3). | | | Catalog # | M1203-04E | | Applications | Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:2000 | | Immunohistochemistry (Paraffin): 1:100 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | N/A | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Rat | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2., 0.1% sodium azide. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Recombinant corresponding to rat MCP-1 expressed in E. coli. Cellular Localization: Secreted. | | Specificity | Recognizes rat MCP-1. Species Crossreactivity: mouse JE. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -Macrophage, Monocyte Chemotactic Protein-1 (MCP-1, Chemokine C-C Motif Ligand 2, CCL2, GDCF-2, HC11, HSMCR30, JE, MGC9434, Monocyte Chemoattractant Protein 1, Monocyte Chemotactic and Activating Factor, Monocyte Chemotactic Protein 1, Monocyte Secretory P |
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