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You are here:Home » Antibodies » Abs to Cell Markers » Anti -Macrophages (Biotin)

Anti -Macrophages (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC
The antibody is associated with fully differentiated tissue macrophages both in normal and in diseased tissues, independently of the presence or absence of inflammation. The antigen is absent on freshly isolated monocytes and other blood cells. After 6 to 7 days culture human monocytes become positive. Some melanoma and carcinoma cell lines are also positive.
Catalog #M1250-01A
ApplicationsSuitable for use in Western Blot, Flow Cytometry, Immunohistochemistry. Useful for Macrophage Phenotyping. Other applications not tested.
Recommended DilutionsWestern Blot: 1:50
Flow Cytometry: 1:50
Immunohistochemistry (Frozen, paraffin): 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No9G224
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide. Labeled with Biotin.
PurityPurified by Protein G affinity chromatography.
SpecificityRecognizes a protein of 86kD on the cell surface and within the cytoplasm of mature macrophages. Species Crossreactivity: human, a subpopulation of macrophages of rhesus monkey, pig alveolar macrophages and Kupffer cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names86 kD ag


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