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You are here:Home » Antibodies » Abs to Cell Markers » Anti -Macrophages (Tissue And Proliferative Disorders)

Anti -Macrophages (Tissue And Proliferative Disorders)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Macrophages and histiocytes, which are widely distributed throughout the body, play a crucial role in the body defense mechanisms. They are heterogenous in morphology, cytochemistry, function and surface antigens. Immunohistochemical labeling with monoclonal antibodies is one of the most specific and reliable techniques for the identification and localization of these cells. Monoclonal antibodies to macrophage and histiocyte surface or cytoplasmic antigens are important for the immunodiagnosis of both benign and malignant histiocytic lesions. They are useful in the investigation of macrophage differentiation and maturation and in the diagnosis of various inflammatory, reactive and neoplastic disorders of macrophages.
Catalog #M1250-15
ApplicationsSuitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Frozen, acetone): 1:200-1:800
Note: Epitope is sensitive to formaldehyde fixation.
Western Blot: Recognizes an antigen of 150kD on macrophage cell membranes.
Optimal dilutions to be determined by the researcher.
Immunohistochemistry Positive Control TissueTonsil
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10F26
HostMouse
SourceHuman
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.2, 1% BSA, 0.01% thimerosal. Reconstitute with 500ul sterile ddH2O.
PurityPurified by affinity chromatography
ImmunogenCultured human peritoneal macrophages.
SpecificityRecognizes most human tissue macrophages in thymus, spleen, lymph nodes and tonsil. It is positive on Kupffer cells (liver), alveolar macrophages and macrophages in the interstitial tissue of the kidney, pancreas and other organs. Does not react with macrophage scavenger receptors AI and AII. Species Crossreactivity: canine, porcine, monkey, feline and bovine. Does not react with hamster, guinea pig, equine, rat, goat or rabbit
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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