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Anti -Macrophages, Late Stage Inflammatory
Anti -Macrophages, Late Stage Inflammatory
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IH FC |
|
| The antibody is associated with fully differentiated tissue macrophages both in normal and in diseased tissues, independently of the presence or absence of inflammation. The antigen is absent on freshly isolated monocytes and other blood cells. After 6 to 7 days culture human monocytes become positive. Some melanoma and carcinoma cell lines are also positive. This clone is very useful for macrophage phenotyping. | | | Catalog # | M1251-01 | | Applications | Can be used for Western Blot, Flow Cytometry, Immunohistochemistry | | Recommended Dilution | Immunohistochemistry: 1:10 (frozen and paraffin sections) | | Flow Cytometry: 1:10 | | Western Blot: 1:10 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 6A596 | | Host | Mouse | | Source | Human | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, 0.02% sodium azide, 0.1% BSA. | | Purity | Purified IgG | | Specificity | Recognizes a protein of 86kD on the cell surface and within the cytoplasm of mature macrophages. Crossreacts with a subpopulation of macrophages of rhesus monkey, porcine alveolar macrophages and Kupffer cells. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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