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You are here:Home » Antibodies » Abs to Cells, Blood Components » Anti -Macrophages, Late Stage Inflammatory

Anti -Macrophages, Late Stage Inflammatory

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC
The antibody is associated with fully differentiated tissue macrophages both in normal and in diseased tissues, independently of the presence or absence of inflammation. The antigen is absent on freshly isolated monocytes and other blood cells. After 6 to 7 days culture human monocytes become positive. Some melanoma and carcinoma cell lines are also positive. This clone is very useful for macrophage phenotyping.
Catalog #M1251-01
ApplicationsCan be used for Western Blot, Flow Cytometry, Immunohistochemistry
Recommended DilutionImmunohistochemistry: 1:10 (frozen and paraffin sections)
Flow Cytometry: 1:10
Western Blot: 1:10
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6A596
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.02% sodium azide, 0.1% BSA.
PurityPurified IgG
SpecificityRecognizes a protein of 86kD on the cell surface and within the cytoplasm of mature macrophages. Crossreacts with a subpopulation of macrophages of rhesus monkey, porcine alveolar macrophages and Kupffer cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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