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You are here:Home » Antibodies » Abs to Cell Markers » Anti -MAdCAM-1 (Mucosal Addressin Cell Adhesion Molecule 1)

Anti -MAdCAM-1 (Mucosal Addressin Cell Adhesion Molecule 1)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified B IP IH
Mucosal addressin cell adhesion molecule-1 (MadCAM-1) is a cell-surface immunogloblin (Ig) superfamily member composed of two extracellular Ig domains, followed by a mucin-like domain, a transmembrane domain, and a short cutoplasmic domain. The molecule is similar to ICAM1 and VCAM1. It interacts via its N-terminal Ig domain with the lymphocyte homing receptor alpha4 beta73, which plays a critical role in forming the gut-associated lymphoid system. MAdCAM-1 promotes the adhesion of T and B cells, monocytes/macrophages, and potentially eosinophils, basophils, and differentiated mast cells to the vascular endothelium. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene. The molecular weight is about 43kD, and it is highly expressed on high endothelial venules(VEC) and lamina propia venules found in the small intestine.
Catalog #M1275
Fusion Partners Spleen cells from immunized Wistar were fused with cells of a rat yeloma cell line.
ApplicationsSuitable for use in ELISA, Western Blotting, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry: (frozen)- 5ug/ml
Optimal dilutions to be determined by the researcher.
Recommended Secondary ReagentsGoat Anti Rat IgG: HRP (mouse adsorbed)
Recommended Negative ControlsRat IgG2a Negative Control
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3H2630
HostRat
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenBalb/c mouse endothelial cells.
SpecificityRecognizes mouse mucosal vascular addressin, MAdCAM-1. Binds to the N-terminal Ig domain involved in a4b7 integrin binding. 3H2630 blocks MadCAM-1 dependent binding in vitro.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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