| Mucosal addressin cell adhesion molecule-1 (MadCAM-1) is a cell-surface immunogloblin (Ig) superfamily member composed of two extracellular Ig domains, followed by a mucin-like domain, a transmembrane domain, and a short cutoplasmic domain. The molecule is similar to ICAM1 and VCAM1. It interacts via its N-terminal Ig domain with the lymphocyte homing receptor alpha4 beta73, which plays a critical role in forming the gut-associated lymphoid system. MAdCAM-1 promotes the adhesion of T and B cells, monocytes/macrophages, and potentially eosinophils, basophils, and differentiated mast cells to the vascular endothelium. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene. The molecular weight is about 43kD, and it is highly expressed on high endothelial venules(VEC) and lamina propia venules found in the small intestine. |
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| Catalog # | M1275-02 |
| Applications | Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry and Adhesion Blocking. Other applications have not been tested. |
| Recommended Dilution | ELISA: 0.1-1ug/ml |
| Western Blot: 0.5-1ug/ml |
| Immunofluorescence: 1-2ug/ml |
| Immunohistochemistry: 0.5-5ug/ml |
| Adhesion Blocking: 10ug/ml |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1,k |
| Clone No | 6A109 |
| Host | Mouse |
| Source | Human |
| Concentration | 0.5mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, containing 0.1% sodium azide |
| Purity | Purified by Protein A chromatography |
| Immunogen | Recombinant soluble form of MAdCAM-1-Fc (first Ig domain), which shares 56% identity with mouse and rat. |
| Specificity | Specific for human MAdCAM-1 00(Mucosal addressin cell adhesion molecule. The epitope maps to the first Ig domain of human MAdCAM-1. On Western blots, it identifies a band at 70kD. Reactivity has been confirmed with recombinant human MAdCAM-1by Western blotting and with paraffin-embedded human osteoarthritic synovium by immunohistochemistry. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
