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You are here:Home » Antibodies » Abs to Cell Markers » Anti -MAdCAM-1 (Mucosal Addressin Cell Adhesion Molecule 1)

Anti -MAdCAM-1 (Mucosal Addressin Cell Adhesion Molecule 1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC
Mucosal addressin cell adhesion molecule-1 (MadCAM-1) is a cell-surface immunogloblin (Ig) superfamily member composed of two extracellular Ig domains, followed by a mucin-like domain, a transmembrane domain, and a short cutoplasmic domain. The molecule is similar to ICAM1 and VCAM1. It interacts via its N-terminal Ig domain with the lymphocyte homing receptor alpha4 beta73, which plays a critical role in forming the gut-associated lymphoid system. MAdCAM-1 promotes the adhesion of T and B cells, monocytes/macrophages, and potentially eosinophils, basophils, and differentiated mast cells to the vascular endothelium. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene. The molecular weight is about 43kD, and it is highly expressed on high endothelial venules(VEC) and lamina propia venules found in the small intestine.
Catalog #M1275-03A
ApplicationsSuitable for use in Flow Cytometry, ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:10- 1:100. Use10ul of the suggested working dilution to label 10e6 cells in 100ul.
Immunohistochemistry (paraffin): 1:50. Does not require protein digestion pre-treatment of paraffin embedded sections prior to staining. Does not require antigen retrieval prior to staining of paraffin sections.
Western Blot: Detects a band of approximately 60kD under non-reducing conditions.
Optimal dilutions to be determined by the researcher.
HybridomaP3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6A437
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant soluble MAdCAM-1-fc fusion protein.
SpecificityRecognizes human mucosal addressin cell adhesion molecule 1 (MAdCAM-1). MAdCAM-1 is expressed on high endothelial venules of Peyer's patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria but this clone does not recognize MAdCAM-1 in these tissues. This clone reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognized by the clone. This clone is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7 (in press).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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