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You are here:Home » Antibodies » Abs to Cell Markers » Anti -MAdCAM-1 (Mucosal Addressin Cell Adhesion Molecule 1)

Anti -MAdCAM-1 (Mucosal Addressin Cell Adhesion Molecule 1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B FC
Mucosal addressin cell adhesion molecule-1 (MadCAM-1) is a cell-surface immunogloblin (Ig) superfamily member composed of two extracellular Ig domains, followed by a mucin-like domain, a transmembrane domain, and a short cutoplasmic domain. The molecule is similar to ICAM1 and VCAM1. It interacts via its N-terminal Ig domain with the lymphocyte homing receptor alpha4 beta73, which plays a critical role in forming the gut-associated lymphoid system. MAdCAM-1 promotes the adhesion of T and B cells, monocytes/macrophages, and potentially eosinophils, basophils, and differentiated mast cells to the vascular endothelium. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene. The molecular weight is about 43kD, and it is highly expressed on high endothelial venules(VEC) and lamina propia venules found in the small intestine.
Catalog #M1275-03E
Fusion Partners Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.
ApplicationsSuitable for use in Flow Cytometry, ELISA, Western Blot. Other applications not tested.
Recommended DilutionFlow Cytometry: 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Western Blot: Detects a band of approximately 60kD
Optimal dilutions to be determined by the researcher.
Recommended Secondary ReagentsF(ab')2 rabbit anti mouse IgG:FITC
F(ab')2 rabbit anti mouse IgG:RPE
Recommended Negative ControlsMouse IgG1 Negative Control
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6A439
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
ImmunogenRecombinant soluble MAdCAM-1-fc fusion protein
SpecificityRecognizes human mucosal addressin cell adhesion molecule 1 (MAdCAM-1) a 60kD transmembrane protein that belongs to the immunoglobulin superfamily. MAdCAM-1 is strongly expressed on mucosal lymphoid tissue and high endothelial venules of Peyer's patches. Human MAdCAM-1 interacts with the beta 7 integrin LPAM-1 (alpha4beta7), CD62L, and VLA-4 (alpha4beta1) and is involved in lymphocyte trafficking, this clone recognizes the mucin domain of MAdCAM-1. Does not block the function of MAdCAM-1 (in press).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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