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You are here:Home » Antibodies » Abs to Cell Markers » Anti -MAdCAM-1 (Mucosal Addressin Cell Adhesion Molecule 1) (Azide Free)

Anti -MAdCAM-1 (Mucosal Addressin Cell Adhesion Molecule 1) (Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Purified B IP IH FC
Mucosal vascular addressin-1 (MAdCAM-1) is a type I transmembrane glycoprotein expressed at high levels on high endothelial venules (HEV) of Peyer’s patches and mesenteric lymph nodes, and on flatwalled venules within the gut lamina propria. It is also expressed on sinus-lining cells in the spleen(1-3). The countereceptor or “homing receptor” for MAdCAM-1 is alpha4beta7 integrin (also known as LPAM-1). MAdCAM-1 is also a facultative ligand for CD62L/L-selectin(4, 5). This antibody binds to the first domain of MAdCAM-1 and blocks MAdCAM-1-dependent binding in vitro and lymphocyte homing to Peyer’s patch HEV in vivo(1, 2, 4).
Catalog #M1275AX
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot, Affinity Purification and Blockade of Cellular Binding to MAdCAM-1.
Recommended DilutionImmunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No5K304
HostRat
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS. No preservative added.
PurityPurified
ImmunogenEndothelial cells isolated from BALB/c mouse mesenteric and peripheral lymph nodes
SpecificityRecognizes murine MAdCAM-1 (Mr 58-88kD).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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