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You are here:Home » Antibodies » Abs to Cell Markers » Anti -MAdCAM-1 (Mucosal Addressin Cell Adhesion Molecule 1) (FITC)

Anti -MAdCAM-1 (Mucosal Addressin Cell Adhesion Molecule 1) (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Purified B IP IH FC
Mucosal vascular addressin-1 (MAdCAM-1) is a type I transmembrane glycoprotein expressed at high levels on high endothelial venules (HEV) of Peyer’s patches and mesenteric lymph nodes, and on flatwalled venules within the gut lamina propria. It is also expressed on sinus-lining cells in the spleen.1-3 The countereceptor or “homing receptor” for MAdCAM-1 is alpha4beta7 integrin (also known as LPAM-1). MAdCAM-1 is also a facultative ligand for CD62L/L-selectin.4, 5 This antibody binds to the first domain of MAdCAM-1 and blocks MAdCAM-1-dependent binding in vitro and lymphocyte homing to Peyer’s patch HEV in vivo.1, 2, 4
Catalog #M1275B
Applications• Flow cytometry 6
• Immunohistochemistry (frozen sections) 1, 2
• Immunoprecipitation, western blotting and affinity purification 1, 5
• Blockade of cellular binding to MAdCAM-1 1, 2, 4
Recommended DilutionImmunohistochemistry: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No5K304
HostRat
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.1% sodium azide. Conjugated to FITC.
PurityPurified
ImmunogenEndothelial cells isolated from BALB/c mouse mesenteric and peripheral lymph nodes
SpecificityRecognizes murine MAdCAM-1 (Mr 58-88kD).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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