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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IP IH IC IF

The MAGE-A protein is expressed in many types of tumors including melanoma, breast and lung. However, in normal tissues, it is only present in adult testis and placenta. The family of MAGE-A proteins includes MAGE-1, -2, -3, -4, -6, -10, and -12. MAGE-1, -3, -6 and -10 are able to recognize specific cytolytic T lymphocytes derived from tumors or peripheral blood. Additionally, MAGE-3 shows HLA class II restricted epitopes. Therefore, MAGE-A is regarded as a possible
immunotherapy candidate, with clinical studies currently underway involving immunization with MAGE-1 and -3 peptides.
Catalog #	M1750-01D
Applications	Suitable for use in ELISA, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation and Western Blot. Other applications have not been tested.
Recommended Dilution	ELISA (Native): 0.1-1ug/ml
	Immunocytochemistry: 10ug/ml
	Immunohistochemistry (Frozen sections): 10ug/ml
	Immunofluorescence: 10ug/ml
	Immunoprecipitation (Native): 10ug/ml
	Western Blot: 1-3ug/ml
	Optimal dilutions to be determined by the researcher.
Positive Controls	Human SK-MEL-37 melanoma cell lysates.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	6A111
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	Purified by Protein A chromatography.
Immunogen	Full-length recombinant human MAGE-A
Specificity	Recognizes different members of the human MAGE-A family: MAGE-1, -2, -3, -4, -6, -10 and -12. MAGE-10 has a molecular weight of ~72kD. The rest of the MAGE-A proteins have molecular weights ~45-50kD.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.