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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -MAGE A

Anti -MAGE A

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH IC IF
The MAGE-A protein is expressed in many types of tumors including melanoma, breast and lung. However, in normal tissues, it is only present in adult testis and placenta. The family of MAGE-A proteins includes MAGE-1, -2, -3, -4, -6, -10, and -12. MAGE-1, -3, -6 and -10 are able to recognize specific cytolytic T lymphocytes derived from tumors or peripheral blood. Additionally, MAGE-3 shows HLA class II restricted epitopes. Therefore, MAGE-A is regarded as a possible
immunotherapy candidate, with clinical studies currently underway involving immunization with MAGE-1 and -3 peptides.
Catalog #M1750-01D
ApplicationsSuitable for use in ELISA, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation and Western Blot. Other applications have not been tested.
Recommended DilutionELISA (Native): 0.1-1ug/ml
Immunocytochemistry: 10ug/ml
Immunohistochemistry (Frozen sections): 10ug/ml
Immunofluorescence: 10ug/ml
Immunoprecipitation (Native): 10ug/ml
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlsHuman SK-MEL-37 melanoma cell lysates.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG2a
Clone No6A111
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein A chromatography.
ImmunogenFull-length recombinant human MAGE-A
SpecificityRecognizes different members of the human MAGE-A family: MAGE-1, -2, -3, -4, -6, -10 and -12. MAGE-10 has a molecular weight of ~72kD. The rest of the MAGE-A proteins have molecular weights ~45-50kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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