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You are here:Home » Antibodies » Abs to RNA Processing » Anti -MAGOH (Protein Mago Nashi Homolog, MAGOHA)

Anti -MAGOH (Protein Mago Nashi Homolog, MAGOHA)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Remains associated with the mRNA after its export to the cytoplasm and require translation of the mRNA for removal. The heterodimer MAGOH-RBM8A interacts with PYM that function to enhance the translation of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex.
Catalog #M2105-51B
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:2000
Western Blot: 1-3ug/ml, observed in nuclear lysates of cell line HeLa on ~17kD bands
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-SLIGLHFKIKPI, from human MAGOH, at C-terminal (NP_002361.1).
SpecificityRecognizes human MAGOH. Species sequence homology: Bovine, canine, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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