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You are here:Home » Antibodies » Abs to Maltose Binding Protein » Anti -Maltose Binding Protein

Anti -Maltose Binding Protein

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP
Through the use of standard molecular biology techniques, a cDNA encoding a protein of interest can be cloned, in-frame, into an expression vector containing the tag sequence. The resultant tagged protein is usually referred to as an epitopetagged protein or a fusion protein. Currently, a variety of expression vectors containing tags are commercially available. Depending on the expression vector selected, these fusion proteins may be expressed in a variety of organisms including bacteria, yeast, insect cells, and mammalian cells. Detection of a tagged protein is facilitated by the use of an antibody directed against the particular tag. This alleviates the need to generate a specific antibody against the protein itself. Therefore, newly identified proteins can be expressed and studied without having to wait for a specific antibody to be generated. Some of the more commonly used fusion tags include: c-Myc, FLAG, GFP, GST, HA, His, and MBP. Maltose binding protein (MBP) vectors are designed for the expression and purification of MBP fusion proteins(13). Generation of the MBP fusion protein is accomplished by fusing a gene of interest to the malE gene of E. coli (2). MBP vectors contain an inducible tac promoter and the malE translation initiation signals which results in high level expression of the resultant MBP fusion protein(1,3). Such fusion proteins bind with high affinity to columns composed of an amylose resin and can be eluted from the column using free maltose. MBP vectors also possess a sequence encoding the recognition site for the protease, factor Xa(1,3). The protease site is positioned in such a manner that factor Xa cleavage can be utilized to separate the protein of interest from MBP.
Catalog #M2155-09
ApplicationsCan be utilized for the detection and purification of MBP-fusion proteins expressed in E. coli. and other hosts. Suitable for use in ELISA, Western Blotting, Immunoprecipitation. Other applications not tested.
Recommended DilutionELISA: 0.11ug/ml
Western Blotting: 12ug/ml
Immunoprecipitation: 5ug/IP reaction
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3G178
HostMouse
Concentration0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant Maltose Binding Protein derived from the malE gene of E. coli.
SpecificitySpecific for the detection of maltose binding protein (MBP).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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