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You are here:Home » Antibodies » Abs to Enzymes, Phosphorylase » Anti -Maltose Phosphorylase (Biotin)

Anti -Maltose Phosphorylase (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Highly Purified E B IH
In enzymology, a maltose phosphorylase (EC 2.4.1.8) is an enzyme that catalyzes the chemical reaction
maltose + phosphate D-glucose + beta-D-glucose 1-phosphate
Thus, the two substrates of this enzyme are maltose and phosphate, whereas its two products are D-glucose and beta-D-glucose 1-phosphate.
This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is maltose:phosphate 1-beta-D-glucosyltransferase. This enzyme participates in starch and sucrose metabolism.
Catalog #M2155-18
ApplicationsSuitable for Immunoblot (Western or Dot Blot), ELISA, Immunomicroscopy and Immunohistochemistry as well as other antibody based assays using streptavidin or avidin conjugates. Other applications not tested.
Recommended DilutionELISA: 1:4000-1:20,000; 1ug of Maltose Phosphorylase was used in a standard capture ELISA with Streptavidin (HRP) S7973-97A and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]), A0030, as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.
LabelBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio10-20 BAC molecules per Goat IgG molecule
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
Concentration~10mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide, 40% glycerol. Labeled with Biotin.
PurityPurified by delipidation, salt fractionation and ion exchange chromatography.
ImmunogenMaltose Phosphorylase (E.coli).
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Maltose Phosphorylase (E.coli).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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