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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IP IH

Munc18 is a cytosolic brain protein of ~67kD which plays a key role in vesicle membrane fusion events with the plasma membrane. Functional interactions have been demonstrated to exist between Munc18, synaptosomal associated protein of 25kD (SNAP-25), synaptobrevin, and syntaxin. Munc18 is the mammalian homologue of the C. elegans gene unc-18. Mutations in Munc18 can result in accumulations of acetylcholine and paralytic phenotype suggestive of a neurotransmitter release defect. Munc18 thus appears to be required by the synaptic vesicle fusion complex. Two Munc18 isoforms have recently been identified. Munc18-1 has been shown to be primarily expressed in brain, while Munc18-2 is expressed in most tissues.
Catalog #	M2197-02
Applications	Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested.
Recommended Dilutions
	Western Blot: 1ug/ml
	Immunohistochemistry: Frozen sections
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide corresponding to aa58-70, V58EDINKRREPIPS70 of human Munc18.
Specificity	Recognizes Munc18 at ~67kD by Western Blot. Species Crossreactivity: mouse and rat
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.